[Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11872 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11997 [Full Text], T7 Transcription Buffer for DNA Template Preparation (10x), Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec098947 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11040 Place blot into 1xPBS to rinse off the methanol Block 1. [Full Text], Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.rec075275 [Full Text], Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.rec089268 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec9287 [Full Text], TE/Lithium Acetate (TE/LiAc) Solution (pH 7.5), Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.rec092304 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec599 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec085696 pH. [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec085555 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8620 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10704 The following transfer buffer recipes are provided to allow preparation of buffers from scratch. [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec085399 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec454 [Full Text], Terminal deoxynucleotidyl transferase buffer (TdTC buffer), Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11486 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec598 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12039 [Full Text], Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.rec093245 1-Step Transfer Buffer is compatible with Power Blotter and other protein semi-dry transfer devices, when they are paired with a suitable high-current power supply. [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11983 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12060 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec683 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11106 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11830 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec557 [Full Text], Transcription Buffer for RNA Polymerase (5x), Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.rec072504 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec106468 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec082792 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec089920 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec57 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11585 Failure to filter can lead to spotting, where tiny dark grains will contaminate the blot during color development. [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12023 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11289 [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec12407 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10725 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec101113 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12095 10x Transfer buffer: For 4 L • 121.1 g Tris base • 576 g glycine • Bring up the volume to 4 L with ddH 2O 1x Transfer buffer: For 1 L • 700 mL cold ddH 2O • 100 mL 10x Transfer buffer • 200 mL methanol 20x … [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec12470 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11092 [Full Text], Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.rec092981 [Full Text], Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.rec104901 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10416 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10521

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