It is done by taking 5 mL of urine and adding ten drops of concentrated ammonia water and solid ammonium sulfate. ROTHERA'S TEST ON MILK The Diagnostic Value of Rothera's Test on Milk A.B. Place 5 to 10 ml of clear urine in test tube; Boil the upper portion over a flame. Principle of Rothera’s Test Acetoacetic acid and acetone react with alkaline solution of sodium nitroprusside to form a purple colored complex. 2. Rothera's test for acetone. Rothera's test (roth-er-ăz) n. a method of testing urine for the presence of acetone or acetoacetic acid – a sign of diabetes mellitus. The Rothera nitroprusside test looks for ketone bodies in urine. [ A. C. H. Rothera (1880–1915), Australian biochemist] Source for information on Rothera's test: A Dictionary of Nursing dictionary. Paterson, Veterinary Laboratory, Ministry of Agriculture, Weybridge. it is so then glacial acetic acid clear up the turbidity .if it is due to protein then precipitation will be there after the addition of acetic acid Gerhard's test for diacetic acid. 4. Veterinary Laboratory, Ministry of Agriculture Weybridge REFERENCES (1) A.C.H. rothera's test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range - test for ketone bodies. May also be applied to milk. Rothera's test: a test for the presence of ketone bodies, diacetic acid and acetone in urine. Rothera, J. Physiol xxxii: (1908) 491-(2) Harding, Ruttan, Biochem. Updates Video Tutorials Practical Videos (MBBS+BDS+DMLT) Rothera's Test || Rothera's Test for Ketone Bodies || Biochemistry Practical Video Tutorials Practical Videos (MBBS+BDS+DMLT) Rothera's Test || Rothera's Test for Ketone Bodies || Biochemistry Practical Han’s method for betahydroxybutyric acid. Principle. Lindeman's test for diacetic acid. 5. Tablet test. Rothera's test. If turbidity develops add 1-2 drops of glacial acetic acid. Sometimes turbidity may be due to phosphate or carbonate precipitation. 3. Quick Reference (roth-er-ăz) a method of testing urine for the presence of acetone or acetoacetic acid – a sign of diabetes mellitus.

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